百蕊草亲水性化学成分研究
投稿时间:2017-12-04  修订日期:2018-01-17  点此下载全文
引用本文:宣伟东,范正平,胡水根,徐德东.百蕊草亲水性化学成分研究[J].药学实践杂志,2018,36(3):270~273
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作者单位
宣伟东 解放军411医院药学科, 上海 200081 
范正平 解放军411医院药学科, 上海 200081 
胡水根 解放军411医院药学科, 上海 200081 
徐德东 解放军411医院药学科, 上海 200081 
基金项目:上海市卫生局局级科研课题(2012085)
中文摘要:目的 对百蕊草中亲水性化学成分进行研究。方法 采用硅胶柱色谱、SephadexLH-20凝胶柱色谱等分离手段,对百蕊草水提取物经AB-8大孔树脂吸附,50%乙醇洗脱的亲水性组分(TT50)进行分离纯化,应用核磁共振波谱分析、结合文献报道鉴定化合物结构。结果 从TT50中分离纯化得到6个化合物,分别鉴定为:山奈酚(1),紫云英苷(2),山奈酚-3,7-二-O-β-D-吡喃葡萄糖苷(3),山奈酚-3-O-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷(4),山奈酚-3-O-L-吡喃鼠李糖基(1→2)-[6-O-乙酰基]-β-D-吡喃葡萄糖苷(5),芸香苷(6)。结论 TT50中主要成分为以山奈酚为母核的黄酮苷类化合物,化合物45为首次从百蕊草中分离得到。
中文关键词:百蕊草  化学成分  黄酮苷
 
Study on hydrophilic chemical constituents from Thesium Chinensis Turcz
Abstract:Objective To investigate the hydrophilic chemical constituents from Thesium Chinensis Turcz. Methods The water extract of Thesium Chinensis Turcz. was adsorbed onto AB-8 resin column and eluted with 50% ethanol to give the hydrophilic portion TT50. TT50 was further separated and purified by repeated column chromatography on silica gel,Sephadex LH-20. The structures of these purified compounds were identified by NMR spectral analysis and comparison with the reported data. Results Six compounds were isolated and identified as kaempferol (1),kaempferol-3-O-glucoside (2),kaepmferol-3,7-di-O-β-D-glucopyranoside (3),kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside (4),kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside (5),rutinoside (6). Conclusion The main constituents of TT50 were kaempferol glycosides. Compounds 4 and 5 were isolated from this plant for the first time.
keywords:Thesium chinensis Turcz  chemical constituents  flavonoid glycosides
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