啤酒花活性成分黄腐酚抗骨质疏松作用研究
投稿时间:2018-03-20  修订日期:2018-04-27  点此下载全文
引用本文:林柳悦,夏天爽,蒋益萍,李常惠,秦路平,辛海量.啤酒花活性成分黄腐酚抗骨质疏松作用研究[J].药学实践杂志,2018,36(3):219~223
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作者单位E-mail
林柳悦 福建中医药大学药学院, 福建 福州 350122  
夏天爽 第二军医大学药学院生药学教研室, 上海 200433  
蒋益萍 第二军医大学药学院生药学教研室, 上海 200433  
李常惠 第二军医大学药学院生药学教研室, 上海 200433  
秦路平 福建中医药大学药学院, 福建 福州 350122
第二军医大学药学院生药学教研室, 上海 200433 
qinsmmu@126.com 
辛海量 第二军医大学药学院生药学教研室, 上海 200433 hailiangxin@163.com 
基金项目:国家自然科学基金(U1603283)
中文摘要:目的 对黄腐酚在动物及成骨细胞水平上的抗骨质疏松作用进行评价。方法 采用去卵巢小鼠骨质疏松模型进行体内药效学验证。运用Elisa试剂盒及Micro-CT检测方法,对小鼠血清生化指标及股骨骨密度、骨组织形态学进行评价。同时结合成骨细胞增殖、分化和矿化水平分析,以及骨形成相关蛋白的Western blot检测,对黄腐酚抗骨质疏松作用进行系统评价。结果 在体内药效学研究中,黄腐酚可显著提高去卵巢小鼠雌激素水平,降低高骨转换率;改善骨小梁微环境,增强骨密度。在成骨细胞水平上,黄腐酚既可以促进成骨细胞增殖、碱性磷酸酶(ALP)活性以及骨矿化水平,又可以提高骨桥蛋白(OPN)、骨涎蛋白(BSP)和骨形成蛋白(BMP-2)的表达。结论 本研究首次明确了黄腐酚具有抗骨质疏松作用,为开发治疗骨质疏松的药物提供新的资源。
中文关键词:黄腐酚  抗骨质疏松  去卵巢  成骨细胞
 
Anti-osteoporotic study on xanthohumol, an active ingredient in Humulus lupulus L.
Abstract:Objective To evaluate the anti-osteoporotic effect of xanthohumol (XN) in animal and osteoblast. Methods The anti-osteoporotic study on XN was performed with ovariectomized mice model. Serum biochemical indexes, bone mineral density (BMD) and bone histomorphology were measured using Elisa kits and Micro-CT analysis. In vitro test, the effect of XN on osteoblastic proliferation, differentiation and mineralization were assayed. The expression of protein related to bone formation was measured by Western blot analysis. Results In vivo experiments, XN significantly increased the estrogen level, reduced the high bone turnover rate, improved the microenvironment and BMD in ovariectomized mice. In vitro experiments, XN protected bone loss not only by promoting osteoblastic proliferation, ALP activity and bone mineralization, but also through increasing the expression of osteopontin (OPN), bone sialoprotein (BSP) and bone morphogenetic protein-2 (BMP-2). Conclusion This is the first report to confirm that XN has anti-osteoporotic effect, which provides a new approach for the clinical treatment of osteoporosis.
keywords:xanthohumol  anti-osteoporosis  ovariectomy  osteoblast
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