基于基因芯片技术研究生物黏附材料促进三七皂苷吸收的机制
投稿时间:2017-06-26  修订日期:2018-01-20  点此下载全文
引用本文:黎迎,朱春燕.基于基因芯片技术研究生物黏附材料促进三七皂苷吸收的机制[J].药学实践杂志,2018,36(3):215~218,223
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作者单位
黎迎 中国医学科学院、北京协和医学院药用植物研究所, 北京 100193 
朱春燕 中国医学科学院、北京协和医学院药用植物研究所, 北京 100193 
基金项目:重大协同创新项目重大前沿研究(2016-I2M-1-012)
中文摘要:目的 采用基因芯片技术研究3种生物黏附材料——壳聚糖、卡波姆和羟丙基甲基纤维素(HPMC)促进三七皂苷(PNS)吸收的机制。方法 将药物分为4组,分别是PNS组、PNS-壳聚糖组、PNS-卡波姆组和PNS-HPMC组,各组药物经Caco-2细胞转运后,通过基因芯片分析发生显著性变化的溶质运载蛋白(the solute carrier,SLC)和ABC家族转运蛋白(ATP-binding cassette transporters,ABC)基因,从而探索生物黏附材料对PNS吸收的影响机制。结果 与PNS组相比,壳聚糖和卡波姆均提高了SLC基因的表达水平。壳聚糖降低了多药耐药基因和P-gp外排基因的表达水平。HPMC对SLC和ABC两大蛋白家族基因表达无显著影响。结论 壳聚糖和卡波姆提高PNS的口服生物利用度与SLC和ABC转运蛋白有关。而HPMC对基因变化影响较小,其作用机制可能与延长吸收部位滞留时间有关。
中文关键词:三七皂苷  生物黏附材料  吸收机制  基因芯片技术
 
Study on effect mechanism of bioadhesive polymers on panax notoginseng saponins's absorption by microarray technology
Abstract:Objective To study the mechanism of bioadhesive materials (Chitosan, Carbomer and hydroxypropyl methyl cellulose(HPMC)) on promoting panax notoginseng saponins(PNS)'s oral absorption by microarray. Methods The study was divided into four groups.PNS, PNS-chitosan, PNS-carbomer and PNS-HPMC, microarray were used to investigate the change of genes expression level affiliated to the solute carrier transporter(SLC) and the ATP-binding cassette (ABC) after transporting across Caco-2 cell monolayer, to explore the mechanism of bioadhesive materials' effect on PNS's absorption. Results Comparing with PNS group, chitosan and carbomer could significantly increase gene expression level affiliated to SLC transporter, chitosan could decrease multi-resistant genes and P-gp efflux genes expression level affiliated to ABC transporter. HPMC had no obvious effect on SLC and ABC transporter. Conclusion Chitosan and carbomer increase PNS's oral due to genes change affiliated to SLC and ABC transporter that could promote absorption and inhibit efflux. The promotion mechanism of HPMC absorption was that it could prolong retention time on absorption site.
keywords:panax notoginseng saponins  bioadhesive polymers  absorption mechanism  microarray technology
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