胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究
投稿时间:2017-12-01  修订日期:2018-01-04  点此下载全文
引用本文:黄瑾,刘景雪,曾颖,袁芳.胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J].药学实践杂志,2018,36(2):126~130
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作者单位
黄瑾 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437
上海市浦东新区人民医院药剂科, 上海 201200 
刘景雪 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437 
曾颖 上海市浦东新区人民医院药剂科, 上海 201200 
袁芳 上海市浦东新区人民医院药剂科, 上海 201200 
基金项目:上海市卫生和计划生育委员会科研课题资助项目(201440461),浦东新区卫生系统学科带头人培养计划(PWRd2014-11)
中文摘要:目的 研究TRPV1受体的表达变化对表皮生长因子受体(EGFR)蛋白表达的调控作用,及其对相关细胞功能的影响。方法 利用过表达技术和siRNA技术研究TRPV1表达变化对EGFR蛋白表达的影响,用CCK-8实验检测细胞的增殖,并用Real-time PCR实验对功能相关的癌基因Akt2和K-ras的表达进行检测。结果 在胰腺癌细胞中TRPV1过表达或是受激动剂激活后,EGFR的蛋白含量下调;反之,干扰TRPV1表达会使EGFR的蛋白表达增加;过表达TRPV1的胰腺癌细胞其增殖速率减缓;过表达TRPV1的胰腺癌细胞中原癌基因Akt2和K-ras的表达明显受到抑制,反之则激活其表达。结论 胰腺癌细胞中TRPV1的表达调控了EGFR的蛋白含量,并对与其功能密切相关的细胞增殖和癌基因表达也有调节作用。
中文关键词:胰腺癌细胞  TRPV1  表皮生长因子受体  细胞增殖
 
Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells
Abstract:Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.
keywords:transient receptor potential vanilloid 1  EGFR  pancreatic cancer cells  cell proliferation
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