槲皮素与锌离子结合性质及抗氧化活性研究
投稿时间:2016-12-08  修订日期:2017-04-25  点此下载全文
引用本文:邱丽娟,孟欣,候升书,杨根金,仲维清.槲皮素与锌离子结合性质及抗氧化活性研究[J].药学实践杂志,2018,36(1):50~54
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作者单位E-mail
邱丽娟 第二军医大学基础医学院, 上海 200433  
孟欣 第二军医大学药学院, 上海 200433  
候升书 第二军医大学药学院, 上海 200433  
杨根金 第二军医大学药学院, 上海 200433  
仲维清 第二军医大学药学院, 上海 200433 wqzhong2002@sohu.com 
基金项目:国家自然科学基金(21273283)
中文摘要:目的 研究槲皮素与其锌离子(Zn2+)配合物的抗氧化性能。方法 通过紫外/可见分光光度法测定槲皮素与Zn2+的结合比例及结合常数,结合核磁共振技术确定其结合位点,以1,1-二苯基苯肼(DPPH)为探针,比较研究槲皮素与其Zn2+配合物的抗氧化性能。结果 当槲皮素中低于2个酚羟基被中和时,槲皮素与Zn2+的饱和结合比为2:1;当超过3个酚羟基被中和时,槲皮素与Zn2+的结合比为1:1,此时槲皮素-Zn2+的表观结合常数为2.42×106 M-1。槲皮素的3'-O、4'-O参与了与Zn2+的配位。槲皮素-Zn2+配合物清除DPPH自由基的活性比槲皮素本身高出2.3倍。结论 Zn2+可与槲皮素形成稳定的配合物,且其比槲皮素本身具有更高效的抗氧化性能,这为传统中药槲皮素的应用提供了新思路。
中文关键词:槲皮素  锌离子  结合常数  抗氧化
 
Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities
Abstract:Objective To study the anti-oxidation properties of Zn2+-quercetin complexes. Methods The binding stoichiometric ratios and the binding constant of Zn2+ to quercetin were measured with UV-Vis spectroscopy. The binding sites on quercetin were determined via 1H NMR spectroscopy. The antioxidant activities of Zn2+-quercetin complexes were compared to quercetin by scavenging DPPH radical method. The binding ratio of Zn2+ to quercetin depends on neutralization of phenol groups of quercetin. Results The binding ratio of Zn2+ to quercetin is 1:2 when less than two OH groups were deprotonated. The binding ratio is 1:1 when more than three OH groups were deprotonated. The apparent binding constant is 2.42×106 M-1 for the 1:1 Zn2+-quercetin complex. The 3'-OH and 4'-OH of quercetin are involved in the Zn2+ binding. The scavenging DPPH radical activity of Zn2+-quercetin complex is 2.3 times of quercetin. Conclusion These results provide a new insight to expand applications of this traditional Chinese medicine.
keywords:quercetin  Zn2+ ion  binding constant  antioxidant activity
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