HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量
投稿时间:2017-05-15  修订日期:2017-07-31  点此下载全文
引用本文:王娅俐,彭贤东,郝伟迤,王丹,郭廷东,唐志立.HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量[J].药学实践杂志,2017,35(6):551~553,576
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作者单位E-mail
王娅俐 南充市中心医院药学部, 四川 南充 637000  
彭贤东 南充市中心医院药学部, 四川 南充 637000  
郝伟迤 南充市中心医院药学部, 四川 南充 637000  
王丹 南充市中心医院药学部, 四川 南充 637000  
郭廷东 南充市中心医院药学部, 四川 南充 637000  
唐志立 南充市中心医院药学部, 四川 南充 637000 1003955916@qq.com 
中文摘要:目的 建立以HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚含量的方法。方法 色谱柱为SHIADZU-GL Inertsil® ODS-3 RP C18,流动相为甲醇-水(60:40),检测波长为285 nm,流速为1.0 ml/min,柱温为40℃,外标法定量。结果 醋酸地塞米松、樟脑和苯酚的线性范围分别为4.024~40.24、101.7~2 033和10.38~425.2 μg/ml,相关系数r >0.999 5;三者的平均回收率分别为101.2%(RSD为0.56%)、99.89%(RSD为0.72%)、100.2%(RSD为0.97%);重复性试验中RSD均小于1.5%。结论 本方法简便、准确,重复性好,可用于地米樟酚乳膏的质量控制。
中文关键词:高效液相色谱  乳膏  醋酸地塞米松  樟脑  苯酚  含量测定
 
Simultaneous determination of dexamethasone acetate, camphor and phenol in compound cream by HPLC method
Abstract:Objective To establish a HPLC method for simultaneously determining the content of dexamethasone acetate, camphor and phenol in compound cream.Methods The separation was performed on a SHIADZU-GL Inertsil® ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water (60:40). The flow rate was 1.0 ml/min. The detection wave length was 285 nm and the column temperature was 40℃.Results Dexamethasone acetate,camphor and phenol showed good linearity (r>0.999 5, n=7) within the concentration range of 4.024-40.24,101.7-2 033 and 10.38-425.2 μg/ml,respectively. The average recovery of dexamethasone acetate, camphor and phenol was 101.2%(RSD was 0.56%),99.89%(RSD was 0.72%),100.2%(RSD was 0.97%),respectively. Moreover, the RSDs were less than 1.5% in the repeated tests. Conclusion The method was simple, quick and accurate. It is suitable for the quality control of dexamethasone acetate camphor and phenol cream.
keywords:HPLC  cream  dexamethasone acetate  camphor  phenol  determination
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