AB-8大孔树脂纯化五味子木脂素工艺研究
投稿时间:2016-11-22  修订日期:2017-03-23  点此下载全文
引用本文:蒋益萍,薛黎明,辛海量,张巧艳,秦路平.AB-8大孔树脂纯化五味子木脂素工艺研究[J].药学实践杂志,2017,35(6):520~525
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作者单位E-mail
蒋益萍 第二军医大学药学院生药学教研室, 上海 200433  
薛黎明 上海市疾病预防控制中心化学品毒性检定所, 上海 200336  
辛海量 第二军医大学药学院生药学教研室, 上海 200433  
张巧艳 第二军医大学药学院生药学教研室, 上海 200433 Zqy1965@163.com 
秦路平 第二军医大学药学院生药学教研室, 上海 200433 qinsmmu@126.com 
基金项目:国家自然科学基金资助项目(81403162,81503493)
中文摘要:目的 研究AB-8大孔吸附树脂分离纯化五味子木脂素的工艺条件。方法 采用高效液相色谱法对五味子醇甲、五味子甲素和五味子乙素进行定量分析,通过考察静态和动态吸附、洗脱效果,筛选AB-8大孔吸附树脂分离纯化五味子木脂素的最佳工艺条件,对加样量、上样液浓度、洗脱速度和乙醇浓度进行考察。结果 AB-8大孔树脂分离五味子木脂素的最佳工艺条件为:上样量1 BV(bed volume),上样液质量浓度9.159~16.523 mg/ml,树脂柱径高比1:5,吸附流速2.5 BV/h,吸附后用5 BV的30%乙醇洗脱,再以10 BV的95%乙醇洗脱,纯化处理后五味子木脂素总含量达到22.06%,转移率高达77.07%。结论 AB-8大孔吸附树脂能有效分离纯化五味子木脂素,该方法具有操作简单、成本低廉等突出优点,有较高的工业生产应用价值。
中文关键词:五味子  木脂素  纯化  AB-8大孔树脂
 
Study on the technology for extracting lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous resin
Abstract:Objective To study the conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 type of macroporous adsorption resin.Methods The content of Schizandrol, Deoxyschizandrin, r-Schisandrin was determined by HPLC. The optimum separation and purification process of lignans from schisandra chinensis (Turcz) Baill with AB-8 macroporous adsorption resin were identified by static and dynamic adsorption, desorption tests. The sample volume, concentration, eluting velocity, and ethanol concentration were investigated.Results The optimum conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous adsorption resin were as follows:dosage of sample liquid was 1BV, concentration of sample solution was 9.159-16.523 mg/ml, ratio of diameter to height of resin column 1:5, adsoption flow rate was 2.5 BV/h, eluted by 5 BV 30% ethanol, followed by 10BV 95% ethanol. The transfer rate of lignans was 77.07% with 22.06% of total lignan content. Conclusion AB-8 macroporous adsorptive resin can effectively isolate and purify lignans from schisandra chinensis (Turcz) Baill. This low cost process is easy to operate and with high industrial production value.
keywords:schisandra chinensis (Turcz) Baill  lignans  AB-8 macroporous resin
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