益视明目颗粒质量标准研究
投稿时间:2016-10-16  最后修改时间:2017-01-06  点此下载全文
引用本文:缪婧,童晓东.益视明目颗粒质量标准研究[J].药学实践杂志,2017,35(4):355~358
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作者单位E-mail
缪婧 南通市中医院, 江苏 南通 226001  
童晓东 南通市中医院, 江苏 南通 226001 924822769@qq.com 
中文摘要:目的 建立益视明目颗粒的质量标准。方法 用薄层色谱法(TLC)鉴别制剂中的枸杞子、丹参;用高效液相色谱法(HPLC)定量测定丹参素钠的含量。色谱柱:Kromasil C18柱(4.6 mm×150 mm,5 μm);流速:0.4 ml/min;检测波长:280 nm;柱温:30 ℃;进样量:10 μl;流动相:甲醇-0.5%醋酸水溶液(10∶90)。结果 薄层色谱斑点清晰可见、阴性干扰小,可用于鉴别益视明目颗粒中的枸杞、丹参;丹参素钠在2.00~60.00 μg/ml范围内线性关系良好,r=0.999 7(n=6),平均加样回收率105.6%,RSD=1.60%。结论 建立的方法简便、准确、可靠性高、重现性好,可作为控制益视明目颗粒质量的有效方法。
中文关键词:益视明目颗粒  质量标准  丹参素钠
 
The quality standard research for YI SHI MINGMU granules by HPLC method
Abstract:Objective To establish the quality standard for YI SHI MINGMU granules. Methods TLC method was used to identify Lycium barbarum L and Salvia miltiorrhiza. HPLC method was used to quantitatively analyze the concentration of Tanshinol sodium. The analysis was carried out on a column of Kromasil C18(4.6 mm×150 mm,5 μm)with a mobile phase of methanol and 0.5 % acetic acid at the flow rate of 0.4 ml/min. Column temperature was 30 ℃. The detection wavelength was 280 nm. Results The linearity range of Tanshinol sodium was 2.00~60.00 μg/ml,r2=0.999 7(n=6),with the average recoveries of 105.62%, RSD=1.60%. Conclusion This method is accurate, simple, reliable and reproducible. It can be used for the quality control of YI SHI MINGMU granules.
keywords:YI SHI MINGMU granules  quality standard  Tanshinol sodium
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