HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量
投稿时间:2017-03-02  最后修改时间:2017-05-31  点此下载全文
引用本文:侯晓丽,谭朝丹,时扣荣,顾伟鹰,陈伟成.HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量[J].药学实践杂志,2017,35(4):353~354,378
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侯晓丽 上海中医药大学附属第七人民医院, 上海 200137  
谭朝丹 上海中医药大学附属第七人民医院, 上海 200137  
时扣荣 上海中医药大学附属第七人民医院, 上海 200137  
顾伟鹰 上海中医药大学附属第七人民医院, 上海 200137  
陈伟成 上海中医药大学附属第七人民医院, 上海 200137 wisechn@126.com 
基金项目:上海市进一步加快中医药事业发展三年行动计划项目(ZY3-JSFC-2-1011);上海中医药大学附属第七人民医院"七院新星"人才培养计划(XX2016-10)
中文摘要:目的 建立HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量。方法 采用 Kromasil C18色谱柱(150 mm×4.6 mm,5 μm);流动相:乙腈-0.05 mol/L磷酸二氢钠(用磷酸调节pH=3)(28∶72);流速:1.0 ml/min;柱温:室温(25 ℃);检测波长:345 nm。结果 盐酸小檗碱在1.00~50.00 μg/ml范围内线性关系良好(r=0.999 6),平均回收率为104.70%,RSD为1.60%(n=5)。结论 该方法准确、灵敏度高、专属性强、重现性好,对提高冬柏通淋合剂的质量控制标准具有参考意义。
中文关键词:冬柏通淋合剂  盐酸小檗碱  高效液相色谱
 
Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method
Abstract:Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji. Methods The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used. The mobile phase was acetonitrile and 0.05 mol/L NaH2PO4 solution(pH was adjusted to about 3 with phosphoric acid). The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride. Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6). The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5). Conclusion This method is accurate, sensitive, selective and reproducible. It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.
keywords:Dongbai Tonglin Heji  berberine hydrochloride  HPLC
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