中国西沙群岛沐浴海绵的化学成分研究
投稿时间:2017-01-17  最后修改时间:2017-04-14  点此下载全文
引用本文:田园,徐彬,王景凤,林厚文,杨连娟,杨帆.中国西沙群岛沐浴海绵的化学成分研究[J].药学实践杂志,2017,35(4):315~320,382
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作者单位E-mail
田园 集美大学食品与生物工程学院, 福建 厦门 361021;上海交通大学医学院附属仁济医院药学部, 上海 200127  
徐彬 上海交通大学医学院附属仁济医院药学部, 上海 200127  
王景凤 西部矿业集团有限公司技术研究院, 青海 西宁 810000  
林厚文 上海交通大学医学院附属仁济医院药学部, 上海 200127  
杨连娟 上海市皮肤病医院真菌病科, 上海 200050  
杨帆 上海交通大学医学院附属仁济医院药学部, 上海 200127 yang-fan@sjtu.edu.cn 
基金项目:北京医学奖励基金(YJHYXKYJJ-125);上海市科委科技支撑项目(15431900900)
中文摘要:目的 研究中国西沙群岛沐浴海绵Spongia sp.的化学成分。方法 运用正相硅胶柱色谱、反相ODS柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等多种色谱学分离手段,对沐浴海绵Spongia sp.的石油醚萃取层进行分离纯化;通过理化性质、波谱学数据并结合文献报道鉴定化合物的结构,采用微量稀释法评价化合物的体外抗真菌活性。结果 从海绵Spongia sp.中分离并鉴定了9个化合物,分别为smenodiol(1)、smenospongorine(2)、5-epi-smenospongorine(3)、dictyoceratin C(4)、epi-smenospongidine(5)、 dictyoceratin A(6)、stigmasta-4,6,8(14 ),22-tetraen-3-one(7)、3-oxo-4,6,8(14 )-triunsaturated steroid(8)、ergosta-4,6,8(14 ),22-tetraen-3-one(9)。结论 化合物1 ~ 9均为首次从该属海绵中分离得到,体外抗真菌活性测试显示,化合物 2359 对3种受试菌株(白色念珠菌、须癣毛癣菌和红色毛癣菌)表现出程度不等的抑制活性,MIC值范围在12.5~25 μg/ml。
中文关键词:沐浴海绵  化学成分  倍半萜醌  抗真菌活性
 
Chemical constituents from Spongia sp., a marine sponge in Xisha Islands
Abstract:Objective To investigate the chemical constituents in the marine sponge, Spongia sp., collected from the Xisha Islands. Methods The pure chemical components from the petroleum ether extract of Spongia sp.were obtained by repeated column chromatography on silica gel,ODS,Sephadex LH-20 and semi-preparative HPLC.Their structures were determined by spectroscopic analysis and comparison with the reported data.The antifungal activity of those compounds was evaluated by dilution method. Results 9 compounds were isolated and identified,including smenodiol(1),smenospongorine(2),5-epi-smenospongorine(3),dictyoceratin C(4),epi-smenospongidine(5),dictyoceratin A(6),stigmasta-4,6,8(14),22-tetraen-3-one(7),3-oxo-4,6,8(14)-triunsaturated steroids(8),ergosta-4,6,8(14),22-tetraen-3-one(9). Conclusion Compounds 1 ~ 9 were isolated from the sponge of genus spongia for the first time.Compound 235 and 9 exhibited antifungal activities against Candida albicans,Trichophyton mentaqrophytes and Trichophyton rubrum with the MIC values of 12.5~25 μg/ml.
keywords:Spongia sp.  chemical constituents  sequiterpene quinone  antifungal activity
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