HPLC法测定中药山楂中有效成分绿原酸的含量
投稿时间:2012-06-01  最后修改时间:2012-09-12  点此下载全文
引用本文:梁睿姝,张川,邹豪,金磊.HPLC法测定中药山楂中有效成分绿原酸的含量[J].药学实践杂志,2012,30(6):457~458,477
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作者单位E-mail
梁睿姝 广东双林生物制药有限公司, 广东 湛江 524009  
张川 第二军医大学药学院新药研究中心, 上海 200433  
邹豪 第二军医大学药学院新药研究中心, 上海 200433  
金磊 第二军医大学药学院新药研究中心, 上海 200433 jinl_2001@126.com 
中文摘要:目的 建立测定山楂中绿原酸含量的高效液相色谱(HPLC)法。方法 色谱柱为Sunfire C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.1 mol/L磷酸二氢钠缓冲液(25:75),流速为1.0 ml/min,柱温为28℃,检测器波长为327 nm。结果 绿原酸的质量浓度在7.03~70.3 μg/ml范围内与峰面积值线性关系良好(r2=0.999 6),精密度RSD为0.27%,平均加样回收率为96.92%,RSD为0.92%(n=6)。结论 HPLC法简便易行、结果可靠,可以快速、准确地测定山楂中绿原酸的含量。
中文关键词:绿原酸  高效液相色谱  山楂
 
Determinatin of chlorogenic acid in hawthorn by HPLC
Abstract:Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.
keywords:chlorogenic acid  HPLC  hawthorn
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